Çeşitli hayvan dışkılarında Salmonella etkenlerinin konvansiyonel ve moleküler yöntemlerle saptanması
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Date
2007
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Sağlık Bilimleri Enstitüsü
Abstract
In this study, 388 cloacal swab samples were collected from 109 poultry flocks, 106 bovine faecal samples and 107 faecal samples were collected from 27 sheep herds. Total of 601 faecal samples of different animals were examined for the existence of Salmonella spp. in this study. For the isolation of Salmonella, nonselective preenrichment by TPS was followed by selective enrichment by TTB and plating on selective agar XLT4. Suspect colonies were confirmed biochemically and serologically. Salmonella was isolated in 11(10.09 %) of the 109 broiler flocks tested. Of the 12 (3.09 %) Salmonella strain identified from 388 (10 swabs/sample) poultry cloacal swap samples, 9 (75 %) were detected as serogroup B, and 3(25 %) were detected as serogroup D. From the total of 106 bovine faecal samples, 1 (0.94 %) was isolated as Salmonella serogroup B. Salmonella was not isolated from 107 sheep feacal samples collected from 27 sheep herds. Isolates taken from tetrathionate cultures and determined as Salmonella spp. after biochemical and serological tests were also tested by PCR with primers specifically amplifying 606 bp portion of iroB gene. In all of the Salmonella positive cultures Salmonella spp. specific PCR products were detected. In this study, prevalence of Salmonella strains in animal feacal and cloacal swab samples was determined by using conventional technique. As a result, PCR technique is found to be useful for routine examinations of Salmonella in animal faecal samples since all Salmonella spp. isolated samples were confirmed by PCR.
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Keywords
izolasyon, dk, hayvan, PCR, Salmonella, Animal, isolation, faeces